Journal: Cell Reports

Article Title: MYO6 Regulates Spatial Organization of Signaling Endosomes Driving AKT Activation and Actin Dynamics

doi: 10.1016/j.celrep.2017.05.048

Figure Lengend Snippet: Loss of MYO6 Does Not Affect PI3K Activation or AKT Recruitment to the Plasma Membrane (A) Cells were transfected with GFP-p85, fixed, and stained with anti-EGFR antibody. Images were acquired by confocal microscopy. Scale bars, 10 μm. (B) Box-and-whisker plot of Pearson’s correlation coefficients for colocalization of GFP-p85 with EGFR in experiments shown in (A) (each distribution contains 60 cells from three independent experiments). (C) Cells were transfected with GFP-AKT-PH, starved, and imaged by live-cell TIRF microscopy during addition of EGF (data shown are averages of more than 18 cells per condition from three independent experiments). (D) Cells were transfected with GFP-AKT, starved, and imaged by live-cell TIRF microscopy during addition of EGF (data shown are averages of more than 15 cells per condition from three independent experiments). Error bars shown are SD.

Article Snippet: Lyn-mCherry (Plasmid 38004, deposited by Robin Irvine [ ]), GFP-AKT (Plasmid 39531 deposited by Julian Downward [ ]), GFP-AKT-PH (Plasmid 21218 deposited by Tobias Meyer [ ]), and GFP-p85 (Plasmid 11499 deposited by Ronald Kahn was cloned into pEGFP-C3) were obtained from Addgene.

Techniques: Activation Assay, Clinical Proteomics, Membrane, Transfection, Staining, Confocal Microscopy, Whisker Assay, Microscopy